25-9322-80

antibody from Invitrogen Antibodies
Targeting: ZBTB16 PLZF, ZNF145
Provider product page for 25-9322-80

Antibody data

Product number
25-9322-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
PLZF Monoclonal Antibody (9E12), PE-Cyanine7, eBioscience™
Antibody type
Monoclonal
Antigen
Synthetic peptide
Description
This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF is expressed by innate lymphoid cells and its progenitor cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintenance of homeostatis. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor. This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7. This 9E12 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Isotype
IgG
Antibody clone number
9E12
Vial size
25 µg
Concentration
0.2 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
ZBTB16 protein structure - 25-9322-80 shown in red.