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- Product number
- PA5-81949 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLZF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HYTLDFLSPK TFQQILEYAY TATLQAKAED LDDLLYAAEI LEIEYLEEQC LKMLETIQAS DDNDTEATMA DGGAEEEEDR KARYLKNIFI SKHSSEESGY ASVAGQSLPG PMVDQSPSVS TSFGLSA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Stage-specific embryonic antigen 4 is a membrane marker for enrichment of porcine spermatogonial stem cells.
Zhang P, Li F, Zhang L, Lei P, Zheng Y, Zeng W
Andrology 2020 Nov;8(6):1923-1934
Andrology 2020 Nov;8(6):1923-1934
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PLZF in human Cerebral cortex using PLZF Polyclonal Antibody (Product # PA5-81949) shows moderate nuclear positivity in neuronal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PLZF in human Lung using PLZF Polyclonal Antibody (Product # PA5-81949) shows moderate nuclear positivity in macrophages.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PLZF in human Pancreas using PLZF Polyclonal Antibody (Product # PA5-81949) shows weak nuclear positivity in exocrine glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PLZF in human Testis using PLZF Polyclonal Antibody (Product # PA5-81949) shows moderate nuclear positivity in spermatogonia.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 FIGURE Co-immunostaining of the SSEA4 with VASA, DBA, PLZF, c-KIT, and SOX9 from the 7-d-old (A), 90-d-old (B), and 150-d-old (C) porcine testes. Scale bars = 50 mum
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3 FIGURE SSEA4 was expressed in the undifferentiated spermatogonia. Co-immunofluorescence of SSEA4 and with the undifferentiated spermatogonial marker DBA (A) and PLZF (B) in testicular tissues from different ages. Relative proportion of SSEA4-positive cells co-localized with DBA-positive cells (C) and PLZF-positive cells (D). Data are normalized by the number of double-positive cells and are mean for six independent experiments. NC, Negative Control, staining without primary antibody. Scale bars = 50 mum
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 FIGURE Fluorescence-activated cell sorting (FACS) of the SSEA4-positive populations from the germ cells gate of 90-d-old porcine testes. (A) The FACS process of the SSEA4-positive cells. Left panel: The germ cell gate was evident on the light (forward and side) scatter dot plot from whole population. Middle panel: the SSEA4-positive and SSEA4-negative gate were sorted from germ cell gate. Right panel: the sorted SSEA4-positive cells were assayed by FACS. (B) Analysis of the percentage of PLZF (middle panel) and DBA-positive (right panel) cells in SEA4-positive fraction by FACS and immunocytochemical detection of PLZF/DBA in SEA4-positive fraction. Left panel in B: the negative control staining without the primary antibody. Scale bars = 50 mum
