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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
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Validation data
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- Product number
- MA5-15667 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLZF Monoclonal Antibody (5B3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15667 targets ZBTB16 in IF and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 5B3
- Concentration
- Conc. Not Determined
Submitted references The Cortisol Response of Male and Female Choroidal Endothelial Cells: Implications for Central Serous Chorioretinopathy.
Brinks J, van Dijk EHC, Kiełbasa SM, Mei H, van der Veen I, Peters HAB, Sips HCM, Notenboom RGE, Quax PHA, Boon CJF, Meijer OC
The Journal of clinical endocrinology and metabolism 2022 Jan 18;107(2):512-524
The Journal of clinical endocrinology and metabolism 2022 Jan 18;107(2):512-524
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZBTB16 using ZBTB16 monoclonal antibody (Product # MA5-15667) in HeLa (1) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZBTB16 using ZBTB16 monoclonal antibody (Product # MA5-15667) in HeLa (1) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PLZF Monoclonal Antibody (5B3)(Product # MA5-15667) and bands at 74kDa and 61kDa corresponding to PLZF was observed in LNCaP, HEL 92.1.7 and HeLa, but not in HL-60 and SK-OV-3. Nuclear enriched extracts (30 µg lysate) of LNCaP (Lane 1), HEL 92.1.7 (Lane 2), HeLa (Lane 3), HL-60 (Lane 4), SK-O-V3 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1/1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZBTB16 using ZBTB16 monoclonal antibody (Product # MA5-15667) in HeLa (1) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PLZF was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PLZF Monoclonal Antibody (5B3) (Product # MA5-15667) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using ZBTB16 monoclonal antibody (Product # MA5-15667) (Green). Red: actin filaments have been labeled with phalloidin.
