PA5-29213

antibody from Invitrogen Antibodies

Targeting: ZBTB16 PLZF, ZNF145

Western blot

Antibody data

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Validation data

Product number: PA5-29213 - Provider product page
Provider: Invitrogen Antibodies
Product name: PLZF Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: K562, THP-1. Predicted reactivity: Mouse (97%), Rat (96%), Chicken (85%), Rhesus Monkey (98%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.03 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ZBTB16 protein structure - PA5-29213 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Immortalization and enrichment of porcine SSCs. a The single-cell suspension obtained after the two-enzymatic dissociation. Bar = 100 mum. b The cell fraction after differential plating. Arrows point to the putative germ cells. Bar = 100 mum. c Maps of the vector pLOX-Ttag-iresTK (hereafter referred to as Ttag) and Ef1a-Large T-Ires-Puro (hereafter referred to as Puro). d Left: living staining of Ttag- and Puro-transduced cells for PLD6. Bar = 100 mum; Right: enrichment of PLD6 + cells by FACS. e Images of Ttag- and Puro-transduced cells after sorting. Bar = 100 mum. f Staining of Ttag-transduced PLD6 + cells for lectin DBA, UCHL1, PLZF and VASA. NC: the negative control using the isotype IgG in place of the primary antibody. Bar = 50 mum. g Percentages of sorted cells positive for porcine SSC and germ cell markers. Data are presented as the mean +- SEM of three experiments (technical triplicates)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Additional file 1: Figure S1. Staining of Puro-transduced PLD6 + cells for lectin DBA, UCHL1, PLZF and VASA. NC: the negative control using the isotype IgG in place of the primary antibody. Bar = 50 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5 Purification and characterization of primary SSCs. a Flow cytometry data to illustrate the gating strategy for FACS purification of CDH1 + germ cells. b Immunocytochemical staining of SSC colonies on Sertoli cell feeder layer after 14 days of culture. The fluorescence staining is performed on CDH1 FACS sorted cells following differential plating. Expression of CDH1, Lin28, and PLZF in SSC colonies was showed in green fluorescence. Negative (no primary) control: omission of primary antibody. The nuclei (blue) were stained with Hoechst 33342. Scale bar: 100 mum