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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- IC2944A - Provider product page
- Provider
- R&D Systems
- Product name
- Human PLZF APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human PLZF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZBTB38, rhZNF24, rhZNF143, rhZNF206, rhZNF281, or rhZNF423 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
Q05516- Isotype
- IgG
- Antibody clone number
- 6318100
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references MAIT cells are reduced in frequency and functionally impaired in human T lymphotropic virus type 1 infection: Potential clinical implications.
Acquisition of innate-like microbial reactivity in mucosal tissues during human fetal MAIT-cell development.
TLR-induced cytokines promote effective proinflammatory natural Th17 cell responses.
Human iNKT and MAIT cells exhibit a PLZF-dependent proapoptotic propensity that is counterbalanced by XIAP.
Activation, exhaustion, and persistent decline of the antimicrobial MR1-restricted MAIT-cell population in chronic HIV-1 infection.
Paquin-Proulx D, Greenspun BC, Costa EA, Segurado AC, Kallas EG, Nixon DF, Leal FE
PloS one 2017;12(4):e0175345
PloS one 2017;12(4):e0175345
Acquisition of innate-like microbial reactivity in mucosal tissues during human fetal MAIT-cell development.
Leeansyah E, Loh L, Nixon DF, Sandberg JK
Nature communications 2014;5:3143
Nature communications 2014;5:3143
TLR-induced cytokines promote effective proinflammatory natural Th17 cell responses.
Massot B, Michel ML, Diem S, Ohnmacht C, Latour S, Dy M, Eberl G, Leite-de-Moraes MC
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):5635-42
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):5635-42
Human iNKT and MAIT cells exhibit a PLZF-dependent proapoptotic propensity that is counterbalanced by XIAP.
Gérart S, Sibéril S, Martin E, Lenoir C, Aguilar C, Picard C, Lantz O, Fischer A, Latour S
Blood 2013 Jan 24;121(4):614-23
Blood 2013 Jan 24;121(4):614-23
Activation, exhaustion, and persistent decline of the antimicrobial MR1-restricted MAIT-cell population in chronic HIV-1 infection.
Leeansyah E, Ganesh A, Quigley MF, Sönnerborg A, Andersson J, Hunt PW, Somsouk M, Deeks SG, Martin JN, Moll M, Shacklett BL, Sandberg JK
Blood 2013 Feb 14;121(7):1124-35
Blood 2013 Feb 14;121(7):1124-35
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of PLZF in HL-60 Human Cell Line by Flow Cytometry. HL-60 human acute promyelocytic leukemia cell line was stained with Mouse Anti-Human PLZF APC-conjugated Monoclonal Antibody (Catalog # IC2944A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
