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Western blot
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Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- HPA001600 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA001600, RRID:AB_1078752
- Product name
- Anti-ELK3
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ELK3, Gene description: ELK3, ETS-domain protein (SRF accessory protein 2), Alternative Gene Names: ERP, NET, SAP2, Validated applications: WB, IHC, ICC, ChIP, Uniprot ID: P41970, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references ELK3 Mediated by ZEB1 Facilitates the Growth and Metastasis of Pancreatic Carcinoma by Activating the Wnt/β-Catenin Pathway
ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma
XRP44X, an Inhibitor of Ras/Erk Activation of the Transcription Factor Elk3, Inhibits Tumour Growth and Metastasis in Mice
The Oncogenic MicroRNA Hsa-miR-155-5p Targets the Transcription Factor ELK3 and Links It to the Hypoxia Response
Zhao Q, Ren Y, Xie H, Yu L, Lu J, Jiang W, Xiao W, Zhu Z, Wan R, Li B
Frontiers in Cell and Developmental Biology 2021;9
Frontiers in Cell and Developmental Biology 2021;9
ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma
Liu Z, Ren Z, Zhang C, Qian R, Wang H, Wang J, Zhang W, Liu B, Lian X, Wang Y, Guo Y, Gao Y
Frontiers in Oncology 2021;11
Frontiers in Oncology 2021;11
XRP44X, an Inhibitor of Ras/Erk Activation of the Transcription Factor Elk3, Inhibits Tumour Growth and Metastasis in Mice
Deb S, Semenchenko K, Wasylyk C, Cheung H, Tourrette Y, Maas P, Schalken J, van der Pluijm G, Wasylyk B
PLOS ONE 2016;11(7):e0159531
PLOS ONE 2016;11(7):e0159531
The Oncogenic MicroRNA Hsa-miR-155-5p Targets the Transcription Factor ELK3 and Links It to the Hypoxia Response
Fujii H, Robertson E, Wasylyk C, Ye T, Jung A, Wasylyk B
PLoS ONE 2014;9(11):e113050
PLoS ONE 2014;9(11):e113050
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and ELK3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401602).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-ELK3 (HPA001600, Lot A08304) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
