Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-116187 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SIM1 using the SIM1 Polyclonal Antibody (Product # PA5-116187) at a 1:2000 dilution. A549 whole cell lysates were loaded at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 86 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SIM1 using the SIM1 Polyclonal Antibody (Product # PA5-116187) in 293T cells (lysate at 35 µg) at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SIM1 in paraffin-embedded Human brain tissue using the SIM1 Polyclonal Antibody (Product # PA5-116187) at a dilution of 1:250. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SIM1 in paraffin-embedded Human kidney tissue using the SIM1 Polyclonal Antibody (Product # PA5-116187) at a dilution of 1:250. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9.0). Samples were incubated with primary antibody(1:250) for 15min at room temperature.