AP1410d

antibody from Abcepta

Targeting: TERT EST2, hEST2, TCS1, TP2, TRT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1410d

Provider

Abcepta

Proper citation

Abgent Cat#AP1410d, RRID:AB_1967536

Product name

TERT Antibody (S1125)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.46 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

TERT Antibody (pS1125) (Cat. #AP1410d) western blot analysis in Jurkat cell line and human normal uterus tissue lysates (35ug/lane).This demonstrates the TERT antibody detected the TERT protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of TERT Antibody (S1125)(Cat#AP1410d) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human lymph with TERT Antibody (S1125), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

TERT Antibody (S1125) (Cat. #AP1410d) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50