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LearnNB100-317
antibody from Novus Biologicals
Targeting: TERT
EST2, hEST2, TCS1, TP2, TRT
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-317 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-317, RRID:AB_2201588
- Product name
- Mouse Monoclonal TERT Antibody
- Antibody type
- Monoclonal
- Description
- IgM purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Phase I trial of intravenous Ad5CRT in patients with liver metastasis of gastrointestinal cancers.
Generation of an immortalized human choroid endothelial cell line (iChEC-1) using an endothelial cell specific promoter.
Telomerase Plays a Pivotal Role in Collateral Growth Under Ischemia by Suppressing Age-Induced Oxidative Stress, Expression of p53, and Pro-Apoptotic Proteins.
Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survival.
Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe.
Telomerase reverse transcriptase acts in a feedback loop with NF-κB pathway to regulate macrophage polarization in alcoholic liver disease.
TGF-β/β2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome.
Telomere homeostasis in placentas from pregnancies with uncontrolled diabetes.
Effects of smoking on human telomerase reverse transcriptase expression in the skin.
Universal cancer peptide-based therapeutic vaccine breaks tolerance against telomerase and eradicates established tumor.
Activating enhancer-binding protein-2β nucleolar localization predicts poor survival after stage I non-small cell lung cancer resection.
Synthetic siRNA targeting the breakpoint of EWS/Fli-1 inhibits growth of Ewing sarcoma xenografts in a mouse model.
Interferon-inducible IFI16, a negative regulator of cell growth, down-regulates expression of human telomerase reverse transcriptase (hTERT) gene.
Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues.
Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues.
Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues.
Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis.
The telomerase reverse transcriptase regulates chromatin state and DNA damage responses.
Nucleolin interacts with telomerase.
Telomerase maintains telomere structure in normal human cells.
Telomerase maintains telomere structure in normal human cells.
Lee SJ, Shin SP, Lee SH, Kang JW, Kook MC, Kim IH, Kim HK
Cancer gene therapy 2019 May;26(5-6):174-178
Cancer gene therapy 2019 May;26(5-6):174-178
Generation of an immortalized human choroid endothelial cell line (iChEC-1) using an endothelial cell specific promoter.
Giacalone JC, Miller MJ, Workalemahu G, Reutzel AJ, Ochoa D, Whitmore SS, Stone EM, Tucker BA, Mullins RF
Microvascular research 2019 May;123:50-57
Microvascular research 2019 May;123:50-57
Telomerase Plays a Pivotal Role in Collateral Growth Under Ischemia by Suppressing Age-Induced Oxidative Stress, Expression of p53, and Pro-Apoptotic Proteins.
Kokubun T, Saitoh SI, Miura S, Ishida T, Takeishi Y
International heart journal 2019 May 30;60(3):736-745
International heart journal 2019 May 30;60(3):736-745
Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survival.
Wei J, Yang Q, Shi J, Shi B, Ji M, Hou P
Oncogenesis 2019 Apr 1;8(4):25
Oncogenesis 2019 Apr 1;8(4):25
Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe.
Margalef P, Kotsantis P, Borel V, Bellelli R, Panier S, Boulton SJ
Cell 2018 Jan 25;172(3):439-453.e14
Cell 2018 Jan 25;172(3):439-453.e14
Telomerase reverse transcriptase acts in a feedback loop with NF-κB pathway to regulate macrophage polarization in alcoholic liver disease.
Wu XQ, Yang Y, Li WX, Cheng YH, Li XF, Huang C, Meng XM, Wu BM, Liu XH, Zhang L, Lv XW, Li J
Scientific reports 2016 Jan 4;6:18685
Scientific reports 2016 Jan 4;6:18685
TGF-β/β2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome.
Chen J, Yao ZX, Chen JS, Gi YJ, Muñoz NM, Kundra S, Herlong HF, Jeong YS, Goltsov A, Ohshiro K, Mistry NA, Zhang J, Su X, Choufani S, Mitra A, Li S, Mishra B, White J, Rashid A, Wang AY, Javle M, Davila M, Michaely P, Weksberg R, Hofstetter WL, Finegold MJ, Shay JW, Machida K, Tsukamoto H, Mishra L
The Journal of clinical investigation 2016 Feb;126(2):527-42
The Journal of clinical investigation 2016 Feb;126(2):527-42
Telomere homeostasis in placentas from pregnancies with uncontrolled diabetes.
Biron-Shental T, Liberman M, Elbaz M, Laish I, Sharony R, Amiel A
Placenta 2016 Aug;44:13-8
Placenta 2016 Aug;44:13-8
Effects of smoking on human telomerase reverse transcriptase expression in the skin.
Lotfi RA, El Zawahry KM, Kamar ZA, Hashem Z
International journal of dermatology 2014 Oct;53(10):1205-12
International journal of dermatology 2014 Oct;53(10):1205-12
Universal cancer peptide-based therapeutic vaccine breaks tolerance against telomerase and eradicates established tumor.
Dosset M, Godet Y, Vauchy C, Beziaud L, Lone YC, Sedlik C, Liard C, Levionnois E, Clerc B, Sandoval F, Daguindau E, Wain-Hobson S, Tartour E, Langlade-Demoyen P, Borg C, Adotévi O
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Nov 15;18(22):6284-95
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Nov 15;18(22):6284-95
Activating enhancer-binding protein-2β nucleolar localization predicts poor survival after stage I non-small cell lung cancer resection.
Kim MP, Chen Y, Bekele BN, Lopez A, Khanna A, Chen JQ, Spitz MR, Behrens C, Solis L, Wismach M, Ji L, Wistuba II, Roth JA, Katz RL
The Annals of thoracic surgery 2011 Sep;92(3):1044-50
The Annals of thoracic surgery 2011 Sep;92(3):1044-50
Synthetic siRNA targeting the breakpoint of EWS/Fli-1 inhibits growth of Ewing sarcoma xenografts in a mouse model.
Takigami I, Ohno T, Kitade Y, Hara A, Nagano A, Kawai G, Saitou M, Matsuhashi A, Yamada K, Shimizu K
International journal of cancer 2011 Jan 1;128(1):216-26
International journal of cancer 2011 Jan 1;128(1):216-26
Interferon-inducible IFI16, a negative regulator of cell growth, down-regulates expression of human telomerase reverse transcriptase (hTERT) gene.
Song LL, Ponomareva L, Shen H, Duan X, Alimirah F, Choubey D
PloS one 2010 Jan 5;5(1):e8569
PloS one 2010 Jan 5;5(1):e8569
Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues.
Fabricius EM, Kruse-Boitschenko U, Khoury R, Wildner GP, Raguse JD, Klein M
International journal of oncology 2009 May;34(5):1257-79
International journal of oncology 2009 May;34(5):1257-79
Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues.
Fabricius EM, Kruse-Boitschenko U, Khoury R, Wildner GP, Raguse JD, Klein M, Hoffmeister B
International journal of oncology 2009 Dec;35(6):1377-94
International journal of oncology 2009 Dec;35(6):1377-94
Localization of telomerase hTERT protein in frozen sections of basal cell carcinomas (BCC) and tumor margin tissues.
Fabricius EM, Kruse-Boitschenko U, Khoury R, Wildner GP, Raguse JD, Klein M, Hoffmeister B
International journal of oncology 2009 Dec;35(6):1377-94
International journal of oncology 2009 Dec;35(6):1377-94
Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis.
Zhang Q, Shi S, Liu Y, Uyanne J, Shi Y, Shi S, Le AD
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7787-98
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7787-98
The telomerase reverse transcriptase regulates chromatin state and DNA damage responses.
Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, Ganesan S, Lansdorp PM, Collins K, Hahn WC
Proceedings of the National Academy of Sciences of the United States of America 2005 Jun 7;102(23):8222-7
Proceedings of the National Academy of Sciences of the United States of America 2005 Jun 7;102(23):8222-7
Nucleolin interacts with telomerase.
Khurts S, Masutomi K, Delgermaa L, Arai K, Oishi N, Mizuno H, Hayashi N, Hahn WC, Murakami S
The Journal of biological chemistry 2004 Dec 3;279(49):51508-15
The Journal of biological chemistry 2004 Dec 3;279(49):51508-15
Telomerase maintains telomere structure in normal human cells.
Masutomi K, Yu EY, Khurts S, Ben-Porath I, Currier JL, Metz GB, Brooks MW, Kaneko S, Murakami S, DeCaprio JA, Weinberg RA, Stewart SA, Hahn WC
Cell 2003 Jul 25;114(2):241-53
Cell 2003 Jul 25;114(2):241-53
Telomerase maintains telomere structure in normal human cells.
Masutomi K, Yu EY, Khurts S, Ben-Porath I, Currier JL, Metz GB, Brooks MW, Kaneko S, Murakami S, DeCaprio JA, Weinberg RA, Stewart SA, Hahn WC
Cell 2003 Jul 25;114(2):241-53
Cell 2003 Jul 25;114(2):241-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TERT Antibody (2C4) [NB100-317] - 1:500 dilution on MJ90 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TERT Antibody (2C4) [NB100-317] - Increased levels of IFI16 protein in WI-38 cells reduce hTERT levels and inhibit telomerase activity. Total protein extracts prepared from WI-38 cells, either transfected with control (pCMV) vector (lane 1) or pCMV-IFI16 plasmid (lane 2), were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Human Pancreatic carcinoma showing strong staining of tumor cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.
