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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- AP07776PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP07776PU-N, RRID:AB_1623607
- Product name
- anti TERT (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide -KLH conjugated corresponding to a region aa 1104-1123 near the carboxy terminal end of Human TERT (Accession Number AF018167).
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 50 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Immunoblot using anti-hTERT antibody (1/500 dilution). Endogenous levels of mTERT in NIH 3T3 cells (Lane 1) and hTERT in HeLa cells (Lane 5) are not detectable. After transduction with an hTERT expression vector, both cell types show high levels of hTERT protein (Lanes 2 and 6). SY5Y cells, which have high endogenous levels of hTERT, have detectable hTERT protein in both untransduced (Lane 3) and transduced (Lane 4) cells. The arrow indicates a molecular weight of approximately 127kD.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 3. Immunoprecipitation of hTERT protein from NIH 3T3 cell lysates. The anti-hTERT antibody was used for both Immunoprecipitation and Immunoblotting. Anti-hTERT antibody was able to immunoprecipitate TERT protein from cells with ectopic hTERT expression (Lane 4). The preimmune serum was unable to immunoprecipitate TERT protein (Lanes 1 and 2).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Lung, Bronchiole: Formalin-Fixed Paraffin-Embedded (FFPE) using TERT antibody
