Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB4360 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4360, RRID:AB_1581980
- Product name
- YY1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of YY1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Modulation of YY1 activity by SAP30.
Nuclear factor YY1 inhibits transforming growth factor beta- and bone morphogenetic protein-induced cell differentiation.
Yin Yang 1 is increased in human heart failure and represses the activity of the human alpha-myosin heavy chain promoter.
Huang NE, Lin CH, Lin YS, Yu WC
Biochemical and biophysical research communications 2003 Jun 20;306(1):267-75
Biochemical and biophysical research communications 2003 Jun 20;306(1):267-75
Nuclear factor YY1 inhibits transforming growth factor beta- and bone morphogenetic protein-induced cell differentiation.
Kurisaki K, Kurisaki A, Valcourt U, Terentiev AA, Pardali K, Ten Dijke P, Heldin CH, Ericsson J, Moustakas A
Molecular and cellular biology 2003 Jul;23(13):4494-510
Molecular and cellular biology 2003 Jul;23(13):4494-510
Yin Yang 1 is increased in human heart failure and represses the activity of the human alpha-myosin heavy chain promoter.
Sucharov CC, Mariner P, Long C, Bristow M, Leinwand L
The Journal of biological chemistry 2003 Aug 15;278(33):31233-9
The Journal of biological chemistry 2003 Aug 15;278(33):31233-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of YY1 polyclonal antibody (Cat # PAB4360) in HeLa cell lysate . YY1 (arrow) was detected using purified YY1 polyclonal antibody (Cat # PAB4360) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with YY1 polyclonal antibody (Cat # PAB4360) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)