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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
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- Product number
- 39-0700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MTA1 Monoclonal Antibody (1F3F12)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is specific for the C-terminal region of the human MTA1 protein. On Western blots, it identifies doublets at ~80 kDa. A band of unknown origin is observed at ~150 kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F3F12
- Vial size
- 1 mL
- Concentration
- Conc. Not Determined
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of T47D cells using Zymed Ms anti-MTA1 (Product # 39-0700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of T47D cells using Zymed Ms anti-MTA1 (Product # 39-0700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of MTA1 was achieved by transfecting HeLa with MTA1 specific siRNAs (Silencer® select Product # s17390 and s17389). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the MTA1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with MTA1 Monoclonal Antibody (1F3F12) (Product # 39-0700, 1:750 ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000). Densitometric analysis of this western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MTA1.__13
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-MTA1 Monoclonal Antibody (1F3F12) (Product # 39-0700) and 80 kDa, 50 kDa bands corresponding to MTA1 isoforms along with an uncharacterized band (*) at ~55 kDa was observed across all the cell lines and tissues except mouse kidney. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), T-47D (Lane 3), Mouse Testis (Lane 4), Rat Testis (Lane 5), Mouse Kidney (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:750) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of T47D cells using Zymed Ms anti-MTA1 (Product # 39-0700).
