16821-1-AP

antibody from Proteintech Group

Targeting: SRF MCM1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16821-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16821-1-AP, RRID:AB_2194384

Product name

SRF antibody

Antibody type

Polyclonal

Description

KD/KO validated SRF antibody (Cat. #16821-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Regulatory role of MRTF-A in macrophage polarization and the therapeutic mechanism of Bushen Wenyang Huayu decoction in endometriosis.

Meng X, Qi J, Yang X, Zhang L, Fu X, Li J, Chen J
Journal of ethnopharmacology 2026 Jul 15;366:121602

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Microenvironment-activatable nanoagent for real-time NIR-II monitoring and targeted therapy of arterial restenosis.

Meng X, Song J, Meng H, Liao C, Yin Z, Gong K, Kong D, Qi J, Duan Y
Biomaterials 2026 Jan;324:123482

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The inner nuclear membrane protein LEMD3 organizes the 3D chromatin architecture to maintain vascular smooth muscle cell identity.

Li W, Liao Y, Liu Z, Niu L, Huang J, Jia Y, Xu R, Guan S, Liang Z, Li Y, Wu H, Zhu S, Tan L, Yu F, Wang Z, Sun L, Zhao D, Kong W, Fu Y
Nature communications 2025 Oct 3;16(1):8826

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SRF and CBP jointly regulate integrin β6 overexpression in head and neck squamous cell carcinomas.

Xu M, Luo G, Xiao Y, Zhu F, Yao H, Zhu H, Liu F, Shi S, Deng X
Cellular signalling 2025 Mar;127:111621

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PRMT5 Maintains Homeostasis of the Intestinal Epithelium by Modulating Cell Proliferation and Survival.

Li L, Zhang Z, Wang X, Zhao H, Liu L, Xiao Y, Hua S, Chen YG
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Mar;12(12):e2415559

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Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation.

Yang S, Zhang X, Li X, Li H
Cellular and molecular life sciences : CMLS 2025 Mar 13;82(1):110

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Maternal exposure to polystyrene nanoplastics during gestation and lactation impaired skeletal growth in progeny mice by inhibiting neutrophil extracellular trap formation.

Xiong S, Zhao H, Sun Q, Li X, Qiu H, van Gestel CAM, Cao L, Wang S, Li J, Chen G
Ecotoxicology and environmental safety 2025 Jul 1;299:118355

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TTK Inhibition Alleviates Postinjury Neointimal Formation and Atherosclerosis.

Wu JH, Liu YX, Zong JB, Qiu M, Zhou YF, Li YN, Aili T, Zhao XR, Hu B
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Feb;12(6):e2409250

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SREBP1-mediated lipogenesis promotes dedifferentiation and senescence of vascular smooth muscle cells through epigenetic remodeling.

Li B, Zhou K, Yin A, Liu Y, Wang X, Cui Y, Liu F, Liu Q, Zheng H, Mu Z, Chen K, Wu Y, Chang Y, Bao J
Nature communications 2025 Dec 26;17(1):835

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High ELK3 expression is associated with the wild type IDH1 in glioma and enhances infiltration of M2 macrophages.

Xin Z, Guo Y, Zhou Q, Wang L, Liu W, Jiang X, Ren C
International immunopharmacology 2025 Aug 28;161:115064

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EPC1/2 regulate hematopoietic stem and progenitor cell proliferation by modulating H3 acetylation and DLST.

Liu W, Liu X, Li L, Tai Z, Li G, Liu JX
iScience 2024 Mar 15;27(3):109263

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Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor.

Gong Y, Liu M, Zhang Q, Li J, Cai H, Ran J, Ma L, Ma Y, Quan S
Journal of translational medicine 2024 Jun 12;22(1):561

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A proteome-wide screen identifies the calcium binding proteins, S100A8/S100A9, as clinically relevant therapeutic targets in aortic dissection.

Jiang H, Zhao Y, Su M, Sun L, Chen M, Zhang Z, Ilyas I, Wang Z, Little PJ, Wang L, Weng J, Ge J, Xu S
Pharmacological research 2024 Jan;199:107029

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Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer.

Peng Y, Lei X, Yang Q, Zhang G, He S, Wang M, Ling R, Zheng B, He J, Chen X, Li F, Zhou Q, Zhao L, Ye G, Li G
Experimental & molecular medicine 2024 Feb;56(2):441-452

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MYOCD and SRF-mediated MLCK transcription prevents polymorphonuclear neutrophils from ferroptosis in sepsis-related acute lung injury.

Pan D, Wu Q, Zhang C, Qin T, Jiang T, Wu X, Wu F
Immunologic research 2024 Dec;72(6):1299-1312

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Serum Response Factor Expression in Excess Permits a Dual Contractile-Proliferative Phenotype of Airway Smooth Muscle.

Sun R, Pan X, Ward E, Intrevado R, Morozan A, Lauzon AM, Martin JG
American journal of respiratory cell and molecular biology 2024 Aug;71(2):182-194

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Myocardin regulates fibronectin expression and secretion from human pleural mesothelial cells.

Sakai T, Choo YY, Mitsuhashi S, Ikebe R, Jeffers A, Idell S, Tucker TA, Ikebe M
American journal of physiology. Lung cellular and molecular physiology 2024 Apr 1;326(4):L419-L430

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Total sesquiterpenoids from Eupatorium lindleyanum DC. attenuate bleomycin-induced lung fibrosis by suppressing myofibroblast transition.

Sun X, Zhu M, Xia W, Xu X, Zhang J, Jiang X
Fitoterapia 2023 Sep;169:105567

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Translational landscape of direct cardiac reprogramming reveals a role of Ybx1 in repressing cardiac fate acquisition.

Xie Y, Wang Q, Yang Y, Near D, Wang H, Colon M, Nguyen C, Slattery C, Keepers B, Farber G, Wang TW, Lee SH, Shih YI, Liu J, Qian L
Nature cardiovascular research 2023 Nov;2(11):1060-1077

---

Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB.

Zhou LY, Jin CX, Wang WX, Song L, Shin JB, Du TT, Wu H
eLife 2023 Nov 20;12

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Serum Response Factor-Regulated IDO1/Kyn-Ahr Pathway Promotes Tumorigenesis of Oral Squamous Cell Carcinoma.

Xu M, Zhu F, Yin Q, Yin H, Fang S, Luo G, Huang J, Huang W, Liu F, Zhong M, Deng X
Cancers 2023 Feb 19;15(4)

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Sall1 and Sall4 cooperatively interact with Myocd and SRF to promote cardiomyocyte proliferation by regulating CDK and cyclin genes.

Katano W, Mori S, Sasaki S, Tajika Y, Tomita K, Takeuchi JK, Koshiba-Takeuchi K
Development (Cambridge, England) 2023 Dec 15;150(24)

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Heterogeneity of T cells and macrophages in chlorine-induced acute lung injury in mice using single-cell RNA sequencing.

Zhao CQ, Liu JZ, Liu MM, Ren XT, Kong DQ, Peng J, Cao M, Liu R, Hai CX, Zhang XD
Inhalation toxicology 2022;34(13-14):399-411

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SRSF3 Promotes Angiogenesis in Colorectal Cancer by Splicing SRF.

Chen Y, Yang M, Meng F, Zhang Y, Wang M, Guo X, Yang J, Zhang H, Zhang H, Sun J, Wang W
Frontiers in oncology 2022;12:810610

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Generation and Comparative Analysis of an Itga8-CreER (T2) Mouse with Preferential Activity in Vascular Smooth Muscle Cells.

Warthi G, Faulkner JL, Doja J, Ghanam AR, Gao P, Yang AC, Slivano OJ, Barris CT, Kress TC, Zawieja SD, Griffin SH, Xie X, Ashworth A, Christie CK, Bryant WB, Kumar A, Davis MJ, Long X, Gan L, Belin de Chantemele EJ, Lyu Q, Miano JM
Nature cardiovascular research 2022 Nov;1(11):1084-1100

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Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing.

Huang S, Wang X, Zhang M, Huang M, Yan Y, Chen Y, Zhang Y, Xu J, Bu L, Fan R, Tang H, Zeng C, Zhang L, Zhang L
Stem cell research & therapy 2022 Jun 11;13(1):248

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TPL-2 Inhibits IFN-β Expression via an ERK1/2-TCF-FOS Axis in TLR4-Stimulated Macrophages.

Blair L, Pattison MJ, Chakravarty P, Papoutsopoulou S, Bakiri L, Wagner EF, Smale S, Ley SC
Journal of immunology (Baltimore, Md. : 1950) 2022 Feb 15;208(4):941-954

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Myocardin regulates exon usage in smooth muscle cells through induction of splicing regulatory factors.

Liu L, Kryvokhyzha D, Rippe C, Jacob A, Borreguero-Muñoz A, Stenkula KG, Hansson O, Smith CWJ, Fisher SA, Swärd K
Cellular and molecular life sciences : CMLS 2022 Aug 1;79(8):459

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MicroRNA-125b inhibits the proliferation of vascular smooth muscle cells induced by platelet-derived growth factor BB.

Wang X, Chen S, Gao Y, Yu C, Nie Z, Lu R, Sun Y, Guan Z
Experimental and therapeutic medicine 2021 Aug;22(2):791

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Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling.

Zhang L, Li YM, Zeng XX, Wang XY, Chen SK, Gui LX, Lin MJ
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(2):763-777

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Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes.

Ahmad A, Zhang W, Wu M, Tan S, Zhu T
Genes & genomics 2018 Mar;40(3):243-251

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Targeting AGGF1 (angiogenic factor with G patch and FHA domains 1) for Blocking Neointimal Formation After Vascular Injury.

Yao Y, Hu Z, Ye J, Hu C, Song Q, Da X, Yu Y, Li H, Xu C, Chen Q, Wang QK
Journal of the American Heart Association 2017 Jun 25;6(6)

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microRNA let-7g suppresses PDGF-induced conversion of vascular smooth muscle cell into the synthetic phenotype.

Wang TM, Chen KC, Hsu PY, Lin HF, Wang YS, Chen CY, Liao YC, Juo SH
Journal of cellular and molecular medicine 2017 Dec;21(12):3592-3601

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Pedicled no-touch saphenous vein graft harvest limits vascular smooth muscle cell activation: the PATENT saphenous vein graft study.

Verma S, Lovren F, Pan Y, Yanagawa B, Deb S, Karkhanis R, Quan A, Teoh H, Feder-Elituv R, Moussa F, Souza DS, Fremes SE
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2014 Apr;45(4):717-25

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16821-1-AP(SRF antibody) at dilution of 1:400

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 16821-1-AP(SRF antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The SRF antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SRF. This antibody recognizes human,mouse,rat antigen. The SRF antibody has been validated for the following applications: ELISA, IHC, WB analysis.

Appendix

16821-1-AP.pdf

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