720240

antibody from Invitrogen Antibodies

Targeting: SRF MCM1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

720240

Provider

Invitrogen Antibodies

Product name

SRF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

YTHDC2-Mediated circYTHDC2 N6-Methyladenosine Modification Promotes Vascular Smooth Muscle Cells Dysfunction Through Inhibiting Ten-Eleven Translocation 2.

Yuan J, Liu Y, Zhou L, Xue Y, Lu Z, Gan J
Frontiers in cardiovascular medicine 2021;8:686293

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis was performed on fixed and permeabilized A-431 cells for detection of endogenous SRF using Anti-SRF Rabbit Polyclonal Antibody (Product # 720240, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of SRF protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of SRF. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of endogenous SRF was performed on A-431 cells labeled with Anti-SRF Rabbit Polyclonal Antibody (Product# 720240, 5 ug/ 1M cells) or with Rabbit isotype control and detected with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, (Alexa Fluor® 488 conjugate, Product# A27034, 0.4 ug/ml, 1:2500) as represented by the red and pink histograms respectively. The purple histogram represents unstained control cells and the green histogram represents no-primary-Antibody control. A representative of 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer (4468770).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 CircYTHDC2 negatively regulates TET2. (A) The schematic diagram of TET2-WT or TET2-Mut luciferase reporter gene. The wild type motifs were indicated by green and the mutant motif was indicated by red. A7R5 cells were transfected with TET2-WT or TET2-Mut, combined with circYTHDC2 siRNA. The cells transfected with scramble control were used as control. Luciferase report gene assay were performed to measure the relative luciferase activity. (B) Relative enrichment representing TET2 and circYTHDC2 RNA levels associated with circYTHDC2 junction compared to control. (C) qRT-PCR analysis for the expression of TET2 after high glucose treatment in A7R5 cells. (D) western blotting analysis of TET2 expression after high glucose treatment in A7R5 cells. (E) qRT-PCR analysis for the expression of TET2 after circYTHDC2 siRNA transfection in A7R5 cells. (F) western blotting analysis of TET2 expression after circYTHDC2 siRNA transfection in A7R5 cells. (G) western blotting analysis of TET2 expression after TET2 siRNA transfection in A7R5 cells. (H) western blotting analysis for the expression of MYODC, SRF and KLF4 in A7R5 cells after circYTHDC2 siRNA transfection alone, or combined with TET2 siRNA transfection. (I) qRT-PCR analysis for the expression of TET2 after circYTHDC2 transfection in A7R5 cells. (J) western blotting analysis of TET2 expression after circYTHDC2 transfection in A7R5 cells. (K) western blotting analysis of TET2 expression after TET2 transfection in A7R5 cells.