Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 16821-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SRF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLPTQAGAA AALGRGSALG GSLNRTPTGR PGGGGGTRGA NGGRVPGNGA GLGPGRLERE AAAAAATTPA PTAGALYSGS EGDSESGEEE ELGAERRGLK RSLSEMEIGM VVGGPEASAA ATGGYGPVSG AVSGAKPGKK TRGRVKIKME FIDNKLRRYT TFSKRKTGIM KKAYELSTLT GTQVLLLVAS ETGHVYTFAT RKLQPMITSE TGKALIQTCL NSPDSPPRSD PTTDQRMSAT GFEETDLTYQ VSESDSSGET KDTLKPAFTV TNLPGTTSTI QTAPSTSTTM QVSSGPSFPI TNYLAPVSAS VSPSAVSSAN GTVLKSTGSG PVSSGGLMQL PTSFTLMPG (1-348 aa encoded by BC048211 )
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of SRF antibody (16821-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16821-1-AP (SRF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16821-1-AP (SRF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 16821-1-AP (SRF antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 16821-1-AP (SRF antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SRF (IP:16821-1-AP, 3ug; Detection:16821-1-AP 1:1000) with HeLa cells lysate 3000ug.