V9737

antibody from NSJ Bioreagents

Targeting: SRF MCM1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V9737

Provider

NSJ Bioreagents

Product name

Serum Response Factor Antibody / SRF

Antibody type

Monoclonal

Description

This highly specific Serum Response Factor antibody is suitable for use in ELISA/Immunofluorescence/Western blot/Flow cytometry/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

PCRP-SRF-1F7

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the Serum Response Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of PFA-fixed human HeLa cells using Serum Response Factor antibody (green, clone PCRP-SRF-1F7) and phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon tissue with Serum Response Factor antibody (clone PCRP-SF7-1F1) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS staining of PFA-fixed human HeLa cells with Serum Response Factor antibody (blue, clone PCRP-SRF-1F7) and isotype control (red).

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Serum Response Factor antibody (clone PCRP-SRF-1F7). These results demonstrate the foremost specificity of the PCRP-SRF-1F7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Serum Response Factor antibody (clone PCRP-SRF-1F7) as confirmation of integrity and purity.