MA5-17079

antibody from Invitrogen Antibodies

Targeting: FOXP1 12CC4, hFKH1B, HSPC215, QRF1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17079

Provider

Invitrogen Antibodies

Product name

FOXP1 Monoclonal Antibody (6E4C4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17079 targets FOXP1 in Flow cytometry, pep-ELISA, and WB applications and shows reactivity with Human samples.

Antibody clone number

6E4C4

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FOXP1 was performed by loading 20µg of the indicated whole cell lysates and 5µl of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. FoxP1 was detected at ~75kDa using a FOXP1 mouse monoclonal antibody (Product # MA5-17079) at a dilution of 1:1000 in blocking buffer for one hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:5000 for at least one hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells using FOXP1 monoclonal antibody (Product # MA5-17079) (green) and negative control (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of FOXP1 was done on Jurkat cells. Cells were fixed, permeabilized and stained with a FOXP1 mouse monoclonal antibody (Product # MA5-17079, blue histogram) at a dilution of 1:100. After incubation of the primary antibody on ice for an hour, the cells were stained with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680 conjugate (Product # 35519) at a dilution of 1:50 for at least 30 minutes on ice. A representative 10,000 cells were acquired for each sample. The black histogram represents unstained control cells and the red histogram represents no-primary-antibody control.