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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-52006 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QMQQLQQQHL LSLQRQGLLT IQPGQPALPL QPLAQGMIPT ELQQLWKEVT SAHTAEETTG NNHSSLDLTT TCVSSSAPSK TSLIMNPHAS TNGQLSVHTP KRESLSHEEH PHSHPLYGHG VCKWPGCEAV CEDFQSFLKH LNS Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 97%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.15 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FOXP1 in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a FOXP1 Polyclonal Antibody (Product # PA5-52006).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of FOXP1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FOXP1 Polyclonal Antibody (Product # PA5-52006) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 63X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous FOXP1 protein using FOXP1 Antibody: ChIP was performed using Anti-FOXP1 Polyclonal Antibody (Product # PA5-52006, 2.5 µg) on sheared chromatin from LnCaP cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to PSA, and HSP90AB1 (Active) and SAT2 satellite repeats (Inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
