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- Immunocytochemistry [5]
- Immunohistochemistry [5]
- Other assay [2]
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- Product number
- PA5-88084 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nrf2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: A-549, HepG2, HeLa, Rat liver; Cellular Location: Cytoplasm, Nucleus, cytosol Immunogen sequence: YGDTLLGLSD SEVEELDSAP GSVKQNGPKT PVHSSGDMVQ PLSPSQGQST HVHDAQCENT PEKELPVSPG HRKTPFTKDK HSSRLEAHLT RDELRAKALH IPFPVEKIIN LPVVDFNEMM SKEQFNEAQL ALIRDIRRRG KNKVAAQNCR KRKLENIVEL EQDLDHLKDE KEKLLKEKGE NDKSLHLLKK QLSTLYLEVF SMLRDEDGKP YSPSEYSLQQ TRDGNVFLVP KSKKPDVKKN
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.92 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome.
Lysosomal nitric oxide determines transition from autophagy to ferroptosis after exposure to plasma-activated Ringer's lactate.
Impact of chronic smoking on traumatic brain microvascular injury: An in vitro study.
Sifat AE, Archie SR, Nozohouri S, Villalba H, Zhang Y, Sharma S, Ghanwatkar Y, Vaidya B, Mara D, Cucullo L, Abbruscato TJ
Fluids and barriers of the CNS 2022 Sep 9;19(1):74
Fluids and barriers of the CNS 2022 Sep 9;19(1):74
Lysosomal nitric oxide determines transition from autophagy to ferroptosis after exposure to plasma-activated Ringer's lactate.
Jiang L, Zheng H, Lyu Q, Hayashi S, Sato K, Sekido Y, Nakamura K, Tanaka H, Ishikawa K, Kajiyama H, Mizuno M, Hori M, Toyokuni S
Redox biology 2021 Jul;43:101989
Redox biology 2021 Jul;43:101989
Impact of chronic smoking on traumatic brain microvascular injury: An in vitro study.
Sivandzade F, Alqahtani F, Cucullo L
Journal of cellular and molecular medicine 2021 Aug;25(15):7122-7134
Journal of cellular and molecular medicine 2021 Aug;25(15):7122-7134
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Nrf2 was performed in NIH/3T3 cells using Nrf2 Polyclonal Antibody (Product # PA5-88084).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Nrf2 was performed in PC12 cells using Nrf2 Polyclonal Antibody (Product # PA5-88084).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Nrf2 was performed in HeLa cells using Nrf2 Polyclonal Antibody (Product # PA5-88084).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Nrf2 was performed in NIH/3T3 cells using Nrf2 Polyclonal Antibody (Product # PA5-88084).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Nrf2 was performed in PC12 cells using Nrf2 Polyclonal Antibody (Product # PA5-88084).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nrf2 in paraffin-embedded human liver cancer. Samples were incubated with Nrf2 Polyclonal antibody (Product # PA5-88084) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nrf2 in paraffin-embedded human lung cancer. Samples were incubated with Nrf2 Polyclonal antibody (Product # PA5-88084) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nrf2 in paraffin-embedded mouse brain. Samples were incubated with Nrf2 Polyclonal antibody (Product # PA5-88084) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nrf2 in paraffin-embedded mouse kidney. Samples were incubated with Nrf2 Polyclonal antibody (Product # PA5-88084) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nrf2 in paraffin-embedded rat brain. Samples were incubated with Nrf2 Polyclonal antibody (Product # PA5-88084) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 PAL effects not only through exogenous but also through endogenous nitric oxide ( . NO) on MM cells. (A) Principal components analysis (PCA) of metabolome reveals a separation of main source of variance among initial (cells cultured in complete RPMI1640 medium), control (cells cultured in RL solution), PAL treatment, PAL + Fer-1 and erastin-treatment (N = 3; H290 sarcomatoid MM; 8A, epithelioid MM). (B) Mapping of metabolite levels in citrulline- . NO cycle and urea cycle. Comparison of relative metabolite levels in cells cultivated in complete medium (initial), RL solution (control), PAL, PAL + Fer-1 or erastin. Relative metabolite levels are shown as bar plots. Characteristic reactions of citrulline- . NO cycle and urea cycle are included. (C) Specific PAL-mediated iNOS induction in MM cells. Human mesothelial cells (LP9) and MM cells (H290 and 8A) were treated with PAL (15 min) and then incubated in medium for 0-24 h/37 degC, followed by Western blot analysis. The numbers above the bands indicate relative values by quantification through the entire figures. (D) Biphasic . NO increase. MM cells were treated with or without PAL for 15 min. The cells were then incubated with or without caboxy-PTIO, 1400 W or Fer-1 for an additional 0.5-8 h/37 degC, and . NO was detected by flow cytometry using the DAF-FM DA probe. (E) Association of . NO and iNOS expression. MM cells were treated with PAL (15 min) and then incubated in medium for 0-8 h/37 degC with or without Fer-1/a c
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Brain Nrf2 and ICAM-1 expression in mice after 14 days of JUUL/TS exposure with or without MCAO. A Western blot images of Nrf2 expression in normoxic brain and contralateral & ipsilateral brain hemispheres 24 h after MCAO. B - D Quantification of brain Nrf2 expression normalized to beta-actin and expressed as relative to control (1.0) in normoxic, contralateral, and ipsilateral brain regions. E Western blot images of ICAM-1 expression in normoxic brain and contralateral & ipsilateral brain hemispheres 24 h after MCAO. F - H Quantification of brain ICAM-1 expression normalized to beta-actin and expressed as relative to control (1.0) in normoxic, contralateral, and ipsilateral brain regions. *P < 0.05; n = 9 for each group (B), n = 7, 7, and 6 for control, JUUL, and TS respectively ( C , D ), n = 9 for each group ( F ), n = 6, 5, and 5 for control, JUUL, and TS respectively ( G , H ). Cropped images from blots have been used in some cases to improve the clarity and conciseness of the presentation. The cropped images are delineated with white space; full-length blots developed by X-ray films are presented in Additional file 1 : Fig. S1
