A00078-1

antibody from Boster Biological Technology

Targeting: NFE2L2 NRF2

Provider product page for A00078-1

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [29]
Comments [0]
Validations
Western blot [1]

Submit

Product number: A00078-1 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-NFE2L2 Antibody Picoband™
Antibody type: Polyclonal
Description: Rabbit IgG polyclonal antibody for NFE2L2 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.
Reactivity: Human
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

NFE2L2 protein structure - A00078-1 shown in red.

Submitted references Pterostilbene protects against H(2) O(2) -induced oxidative stress by regulating GAS6/Axl signaling in HL-1 cells.

Di W, Zhao A, Li X, Chen J, Dai Y, Li J, Lei W, Yang Y, Lu H
Cell biochemistry and function 2024 Mar;42(2):e3956

Mitochondrial-targeted curcumin inhibits T-cell activation via Nrf2 and inhibits graft-versus-host-disease in a mouse model.

Patwardhan RS, Gohil D, Singh B, Kumar BK, Purohit V, Thoh M, Checker R, Gardi N, Gota V, Kutala VK, Patwardhan S, Sharma D, Sandur SK
Phytotherapy research : PTR 2024 Mar;38(3):1555-1573

Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway.

Chen W, Zhang K, Cui H, Li J, Yao D, Wang S, Tian X, Kou W, Huang J, Wang H, Wang J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Mar;172:116276

The flower of Abelmoschus manihot (L.) medik exerts antioxidant effects by regulating the Nrf2 signalling pathway in scald injury.

Song Y, Fu Z, Zhu X, Zhang J, Bai W, Song B
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2024 Mar-Apr;32(2):123-134

Daphnetin protects neurons in an Alzheimer disease mouse model and normal rat neurons by inhibiting BACE1 activity and activating the Nrf2/HO-1 pathway.

Zhang X, Ma W, Liu H, Liu Y, Zhang Y, He S, Ding X, Li B, Yan Y
Journal of neuropathology and experimental neurology 2024 Aug 1;83(8):670-683

Untargeted serum and liver metabolomics analyses reveal the gastroprotective effect of polysaccharide from Evodiae fructus on ethanol-induced gastric ulcer in mice.

Luo JH, Zou WS, Li J, Liu W, Huang J, Wu HW, Shen JL, Li F, Yuan JS, Tao AK, Gong L, Zhang J, Wang XY
International journal of biological macromolecules 2023 Mar 31;232:123481

Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments.

Zhang Z, Wang S, Liu X, Yang Y, Zhang Y, Li B, Guo F, Liang J, Hong X, Guo R, Zhang B
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Aug;164:114964

Silibinin exerts neuroprotective effects against cerebral hypoxia/reoxygenation injury by activating the GAS6/Axl pathway.

Li W, Zhang Z, Li J, Mu J, Sun M, Wu X, Niu X, Yang Y, Yan H, Xu X, Xue C, Qian L, Tian Y
Toxicology 2023 Aug 15;495:153598

Tilapia Skin Peptides Ameliorate Cyclophosphamide-Induced Anxiety- and Depression-Like Behavior via Improving Oxidative Stress, Neuroinflammation, Neuron Apoptosis, and Neurogenesis in Mice.

Zhao YT, Yin H, Hu C, Zeng J, Zhang S, Chen S, Zheng W, Li M, Jin L, Liu Y, Wu W, Liu S
Frontiers in nutrition 2022;9:882175

Protection of zero-valent iron nanoparticles against sepsis and septic heart failure.

Wang D, Wang C, Liang Z, Lei W, Deng C, Liu X, Jiang S, Zhu Y, Zhang S, Yang W, Chen Y, Qiu Y, Meng L, Yang Y
Journal of nanobiotechnology 2022 Sep 5;20(1):405

Neuroprotective Effect of Polyphenol Extracts from Terminalia chebula Retz. against Cerebral Ischemia-Reperfusion Injury.

Lin K, Zhou M, Leng C, Tao X, Zhou R, Li Y, Sun B, Shu X, Liu W
Molecules (Basel, Switzerland) 2022 Sep 30;27(19)

Psoralidin protects against cerebral hypoxia/reoxygenation injury: Role of GAS6/Axl signaling.

Lei W, Wu S, Zhao A, Wu Z, Ding B, Yang W, Lu C, Wu X, Li X, Zhang S, Tian Y, Yang Y
Phytotherapy research : PTR 2022 Jun;36(6):2628-2640

Protective effects and mechanisms of lycorine against adriamycin-induced cardiotoxicity.

Wang Z, Chen Y, Gu M, Wu Z, Ding B, Yang W, Wu X, Wang C, Gao X, Yang Y, Yin G
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jul 20;102:154178

Divergent Regulation of Decidual Oxidative-Stress Response by NRF2 and KEAP1 in Preeclampsia with and without Fetal Growth Restriction.

Mundal SB, Rakner JJ, Silva GB, Gierman LM, Austdal M, Basnet P, Elschot M, Bakke SS, Ostrop J, Thomsen LCV, Moses EK, Acharya G, Bjørge L, Iversen AC
International journal of molecular sciences 2022 Feb 10;23(4)

Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism.

Ni HY, Yu L, Zhao XL, Wang LT, Zhao CJ, Huang H, Zhu HL, Efferth T, Gu CB, Fu YJ
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Apr;98:153919

Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells.

Niu X, Cheng Y, Zhang M, Du L, Wu X, Lu C, Li X, Liu S, Zhao A, Zhang S, Wu Z, Ding B, Shi W, Wang C, Yang Y, Tian Y
Frontiers in cell and developmental biology 2021;9:784035

Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.

Li D, Bai X, Jiang Y, Cheng Y
Brain research bulletin 2021 Mar;168:25-35

Maresin conjugates in tissue regeneration-1 suppresses ferroptosis in septic acute kidney injury.

Xiao J, Yang Q, Zhang Y, Xu H, Ye Y, Li L, Yang Y, Jin S
Cell & bioscience 2021 Dec 27;11(1):221

Pterostilbene Exerts Hepatoprotective Effects through Ameliorating LPS/D-Gal-Induced Acute Liver Injury in Mice.

Liu Z, Wang J, Zhang Y, Wu D, Li S, Jiang A, Du C, Xie G
Inflammation 2021 Apr;44(2):526-535

Lactulose and Melibiose Attenuate MPTP-Induced Parkinson's Disease in Mice by Inhibition of Oxidative Stress, Reduction of Neuroinflammation and Up-Regulation of Autophagy.

Lin CH, Wei PC, Chen CM, Huang YT, Lin JL, Lo YS, Lin JL, Lin CY, Wu YR, Chang KH, Lee-Chen GJ
Frontiers in aging neuroscience 2020;12:226

Polysaccharides from Opuntia milpa alta alleviate alloxan-induced INS-1 cells apoptosis via reducing oxidative stress and upregulating Nrf2 expression.

Li W, Lin K, Zhou M, Xiong Q, Li C, Ru Q
Nutrition research (New York, N.Y.) 2020 May;77:108-118

Polyamine analogue QMA attenuated ischemic injury in MCAO rats via ERK and Akt activated Nrf2/HO-1 signaling pathway.

Cen J, Zhao N, Huang WW, Liu L, Xie YY, Gan Y, Wang CJ, Ji BS
European journal of pharmacology 2019 Feb 5;844:165-174

Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro.

Han H, Wang H, Du Y, Gao L
Cell biochemistry and biophysics 2019 Dec;77(4):367-377

Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways.

Cui HX, Chen JH, Li JW, Cheng FR, Yuan K
Molecules (Basel, Switzerland) 2018 Jul 20;23(7)

Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress.

Wang Y, Hai B, Ai L, Cao Y, Li R, Li H, Li Y
Iranian journal of basic medical sciences 2018 Dec;21(12):1238-1244

Procyanidin B2 ameliorates carrageenan-induced chronic nonbacterial prostatitis in rats via anti-inflammatory and activation of the Nrf2 pathway.

Wang W, Chen R, Wang J
Biochemical and biophysical research communications 2017 Nov 4;493(1):794-799

Protection of Nrf2 against arsenite-induced oxidative damage is regulated by the cyclic guanosine monophosphate-protein kinase G signaling pathway.

Chen C, Jiang X, Gu S, Lai Y, Liu Y, Zhang Z
Environmental toxicology 2017 Aug;32(8):2004-2020

MicroRNA-155 regulates arsenite-induced malignant transformation by targeting Nrf2-mediated oxidative damage in human bronchial epithelial cells.

Chen C, Jiang X, Gu S, Zhang Z
Toxicology letters 2017 Aug 15;278:38-47

The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway.

Wang Z, Zhang H, Sun X, Ren L
Molecular medicine reports 2016 Sep;14(3):2389-96

No comments: Submit comment

Supportive validation

Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of NFE2L2 using anti-NFE2L2 antibody (A00078-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2L2 antigen affinity purified polyclonal antibody (Catalog # A00078-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2L2 at approximately 100KD. The expected band size for NFE2L2 is at 100KD.
Additional image