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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA1-38312 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nrf2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is placenta or lung tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 1 mL
- Concentration
- 0.21 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Transcription factor NRF2 uses the Hippo pathway effector TAZ to induce tumorigenesis in glioblastomas.
Transcription factor NFE2L2/NRF2 is a regulator of macroautophagy genes.
Nrf2 deficiency impairs the barrier function of mouse oesophageal epithelium.
Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus.
Escoll M, Lastra D, Pajares M, Robledinos-Antón N, Rojo AI, Fernández-Ginés R, Mendiola M, Martínez-Marín V, Esteban I, López-Larrubia P, Gargini R, Cuadrado A
Redox biology 2020 Feb;30:101425
Redox biology 2020 Feb;30:101425
Transcription factor NFE2L2/NRF2 is a regulator of macroautophagy genes.
Pajares M, Jiménez-Moreno N, García-Yagüe ÁJ, Escoll M, de Ceballos ML, Van Leuven F, Rábano A, Yamamoto M, Rojo AI, Cuadrado A
Autophagy 2016 Oct 2;12(10):1902-1916
Autophagy 2016 Oct 2;12(10):1902-1916
Nrf2 deficiency impairs the barrier function of mouse oesophageal epithelium.
Chen H, Hu Y, Fang Y, Djukic Z, Yamamoto M, Shaheen NJ, Orlando RC, Chen X
Gut 2014 May;63(5):711-9
Gut 2014 May;63(5):711-9
Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus.
Chen H, Li J, Li H, Hu Y, Tevebaugh W, Yamamoto M, Que J, Chen X
PloS one 2012;7(5):e36504
PloS one 2012;7(5):e36504
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Nrf2 using a polyclonal antibody (Product # PA1-38312).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Esophageal hyperkeratosis due to Nrf2 superactivation in Keap1 -/- esophagus. P7 esophagi of a wild-type mouse (A), a Nrf2 -/- mouse (B), a Keap1 -/- mouse (C), and a Nrf2 -/- Keap1 -/- mouse (D), were stained for H&E. Expression of Nrf2 (E-G), Ppargamma/delta (H-J) and pAkt (K-L) were shown in the esophagi of P7 wild-type mouse, P7 Keap1 -/- mouse and adult wild-type mouse. Size bar = 50 um.
