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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-47091 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZBTB17 Recombinant Rabbit Monoclonal Antibody (HL1548)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Store as concentrated solution. Centrifuge briefly prior to opening vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1548
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZBTB17 in Various whole cell extracts (30 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with ZBTB17 Monoclonal antibody (Product # MA5-47091) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. The signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZBTB17 in U2OS cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Miz1 stained by Miz1 monoclonal antibody (Product # MA5-47091) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000.
