Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-1069 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1069, RRID:AB_2293750
- Product name
- Goat Polyclonal E74 like factor 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Regulation of the Ets-related transcription factor Elf-1 by binding to the retinoblastoma protein.
Regulation of the Ets-related transcription factor Elf-1 by binding to the retinoblastoma protein.
Wang CY, Petryniak B, Thompson CB, Kaelin WG, Leiden JM
Science (New York, N.Y.) 1993 May 28;260(5112):1330-5
Science (New York, N.Y.) 1993 May 28;260(5112):1330-5
Regulation of the Ets-related transcription factor Elf-1 by binding to the retinoblastoma protein.
Wang CY, Petryniak B, Thompson CB, Kaelin WG, Leiden JM
Science (New York, N.Y.) 1993 May 28;260(5112):1330-5
Science (New York, N.Y.) 1993 May 28;260(5112):1330-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: E74 like factor 1 Antibody [NB100-1069] - (0.3ug/ml) staining of Human Lymph Node (A), Human Tonsil (B) and Mouse Thymus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: E74 like factor 1 Antibody [NB100-1069] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.