PAB28536

antibody from Abnova Corporation

Targeting: NFE2 NF-E2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28536

Provider

Abnova Corporation

Product name

NFE2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NFE2.

Antigen sequence

SRNRVIQLSTSELGEMELTWQEIMSITELQGLNAP
SEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPL
PPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLS
GLLSEPLQDPLALLDIGLPAGPPKPQEDPES


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with NFE2 polyclonal antibody (Cat # PAB28536) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with NFE2 polyclonal antibody (Cat # PAB28536) at 1-4 ug/ml shows positivity in nucleus but not nucleoli and cytoskeleton (actin filaments).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human NFE2 polyclonal antibody (Cat # PAB28536) shows strong nuclear positivity in bone marrow poietic cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)