GTX102698

antibody from GeneTex

Targeting: NFE2 NF-E2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX102698

Provider

GeneTex

Proper citation

GeneTex Cat#GTX102698, RRID:AB_1950992

Product name

NF-E2 antibody [N1C3]

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Resources for the Comprehensive Discovery of Functional RNA Elements.

Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: 293T 10% SDS PAGE GTX102698 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: mouse spleen 10% SDS PAGE GTX102698 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NF-E2 antibody [N1C3] detects NF-E2 protein at nucleus by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NF-E2 protein stained by NF-E2 antibody [N1C3] (GTX102698) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.