H00004778-M01

antibody from Abnova Corporation

Targeting: NFE2 NF-E2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004778-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004778-M01, RRID:AB_1136969

Product name

NFE2 monoclonal antibody (M01), clone 2C6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant NFE2.

Antigen sequence

MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSIT
ELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSI
HPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIP
VSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQE
DPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEM
YPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSG
PVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNL
PVDDFNELLARYPLTESQLALVRDIRRRGKNKVAA
QNCRKRKLETIVQLERELERLTNERERLLRARGEA
DRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEY
ALQQAADGTIFLVPRGTKMEATD


Isotype

IgG

Antibody clone number

2C6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NFE2 expression in transfected 293T cell line by NFE2 monoclonal antibody (M01), clone 2C6.Lane 1: NFE2 transfected lysate(41.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NFE2 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol