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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-27762 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFE2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, mouse spleen. Predicted reactivity: Mouse (89%), Rat (91%), Pig (91%), Rhesus Monkey (97%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.32 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NF-E2 using 50 µg of mouse spleen lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a NF-E2 polyclonal antibody (Product # PA5-27762) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NF-E2 using 30 µg of 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a NF-E2 polyclonal antibody (Product # PA5-27762) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFE2 Polyclonal Antibody detects NF-E2 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NF-E2 protein stained by NFE2 Polyclonal Antibody (Product # PA5-27762) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
