MAB8167

antibody from Novus Biologicals

Targeting: NKX2-2 NKX2.2, NKX2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB8167

Provider

Novus Biologicals

Product name

Mouse Monoclonal NKX2.2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human NKX2.2 in ELISA and Western Blot.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Integrator of Stress Responses Calmodulin Binding Transcription Activator 1 (Camta1) Regulates miR-212/miR-132 Expression and Insulin Secretion.

Mollet IG, Malm HA, Wendt A, Orho-Melander M, Eliasson L
The Journal of biological chemistry 2016 Aug 26;291(35):18440-52

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse NKX2.2 by Western Blot. Western blot shows lysates of A-673 human Ewing's sarcoma cell line and beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NKX2.2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.