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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-30862 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Nkx2.2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-30862 targets Nkx2.2 in WB applications and shows reactivity with Human samples. The PA5-30862 immunogen is recombinant fragment corresponding to a region within amino acids 60 and 273 of Human Nkx2.2.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.79 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nkx2.2 using 30µg of MCF-7 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Nkx2.2 polyclonal antibody (Product # PA5-30862) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nkx2.2 showing staining in the nucleus of SKNSH cells. SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Nkx2.2 polyclonal antibody (Product # PA5-30862) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
