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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- MA5-56409 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nkx2.2 Recombinant Rabbit Monoclonal Antibody (JE60-66)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE60-66
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Nkx2.2 with C6 cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with Nkx2.2 Recombinant Monoclonal Antibody (Product # MA5-56409)at a dilution of 1:100 in 1% BSA in PBST overnight at 4 ℃. Followed by Goat Anti-Rabbit IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nkx2.2 with frozen mouse E14.5 embryo tissue . The tissues were blocked in 10% negative goat serum (1 hr, room temp) and washed with PBS. Then incubated with Nkx2.2 Recombinant Monoclonal Antibody (Product # MA5-56409) at a dilution of 1:500 (overnight, 4°C) and washed with PBS. Followed by Goat Anti-Rabbit IgG H&L 488 at 1:1,000 dilution. DAPI was used to stain the cell nuclei (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nkx2.2 with paraffin-embedded human pancreas tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with Nkx2.2 Recombinant Monoclonal Antibody (Product # MA5-56409) at a dilution of 1:1,000 (1 hr, room temp).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nkx2.2 with paraffin-embedded mouse pancreas tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with Nkx2.2 Recombinant Monoclonal Antibody (Product # MA5-56409) at a dilution of 1:1,000 (1 hr, room temp).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nkx2.2 with paraffin-embedded rat brain tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with Nkx2.2 Recombinant Monoclonal Antibody (Product # MA5-56409) at a dilution of 1:200 (1 hr, room temp).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nkx2.2 with paraffin-embedded human skeletal muscle tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with Nkx2.2 Recombinant Monoclonal Antibody (Product # MA5-56409) at a dilution of 1:1,000 (1 hr, room temp).
