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antibody from Invitrogen Antibodies
Targeting: SMARCA2
BAF190, BRM, hBRM, hSNF2a, SNF2, SNF2L2, SNF2LA, Sth1p, SWI2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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- Product number
- PA5-78170 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: 293T, A431, HeLa, HepG2
- Concentration
- 0.89 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCA2 in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with SMARCA2 polyclonal antibody (Product # PA5-78170) using a dilution of 1:100 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCA2 in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with SMARCA2 polyclonal antibody (Product # PA5-78170) using a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SMARCA2 in HeLa cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with SMARCA2 polyclonal antibody (Product # PA5-78170) using a dilution of 1:100, followed by phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMARCA2 Polyclonal Antibody detects SMARCA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA2 stained by SMARCA2 Polyclonal Antibody (Product # PA5-78170) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMARCA2 Polyclonal Antibody detects SMARCA2 protein at nucleolus by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA2 stained by SMARCA2 Polyclonal Antibody (Product # PA5-78170) diluted at 1:500. Red: Tau, a marker, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMARCA2 Polyclonal Antibody detects SMARCA2 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. SMARCA2 Polyclonal Antibody (Product # PA5-78170) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMARCA2 Polyclonal Antibody detects SMARCA2 protein at nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. SMARCA2 Polyclonal Antibody (Product # PA5-78170) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
