NB100-55309
antibody from Novus Biologicals
Targeting: SMARCA2
BAF190, BRM, hBRM, hSNF2a, SNF2, SNF2L2, SNF2LA, Sth1p, SWI2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
Reference
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- Product number
- NB100-55309 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55309, RRID:AB_838897
- Product name
- Rabbit Polyclonal BRM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRM Antibody [NB100-55309] - Detection of Human SMARCA2/BRM on HeLa whole cell lysate using NB100-55307. SMARCA2/BRM was also IPed by rabbit anti-SMARCA2/BRM antibodies NB100-55308 and NB100-55309.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- QcWestern: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.