NB100-55308

antibody from Novus Biologicals

Targeting: SMARCA2 BAF190, BRM, hBRM, hSNF2a, SNF2, SNF2L2, SNF2LA, Sth1p, SWI2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-55308

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-55308, RRID:AB_838899

Product name

Rabbit Polyclonal BRM Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Depletion of histone methyltransferase KMT9 inhibits lung cancer cell proliferation by inducing non-apoptotic cell death.

Baumert HM, Metzger E, Fahrner M, George J, Thomas RK, Schilling O, Schüle R
Cancer cell international 2020;20:52

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BRM Antibody [NB100-55308] - Detection of Human SMARCA2/BRM on HeLa whole cell lysate using NB100-55307. SMARCA2/BRM was also IPed by rabbit anti-SMARCA2/BRM antibodies NB100-55308 and NB100-55309.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BRM Antibody [NB100-55308] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55308 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55309, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).