LS-C369901

antibody from LSBio

Targeting: LCP1 CP64, L-PLASTIN, LC64P, PLS2

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C369901

Provider

LSBio

Product name

LCP1 / L-Plastin Antibody (aa2-627) LS-C369901

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: Plastin-2 antibody at 2 ug/ml. Lane 1: HL-60 whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: HeLa whole cell lysate Lane 6: NIH3T3 whole cell lysate secondary Goat.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: Plastin-2 antibody at 2µg/ml Lane 1: HL60 whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 22 kDa Observed band size: 71 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating LCP1 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of LCP1 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: LCP1 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating LCP1 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of LCP1 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: LCP1 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating LCP1 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of LCP1 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: LCP1 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)