Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 653102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#653102, RRID:AB_2561615
- Product name
- Purified anti-LEF1
- Antibody type
- Monoclonal
- Antigen
- Partial human LEF1 recombinant protein (1-298 aa)
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 15H5A18
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 4° C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-LEF1 (clone 15H5A18) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated