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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
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- Product number
- PA1-24462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LEF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LEF1 in 293T cell lysate. Samples were probed with a LEF1 polyclonal antibody (Product # PA1-24462).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LEF1 in human fetal heart using a LEF1 polyclonal antibody (Product # PA1-24462).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of human fetal heart tissue using LEF1 Polyclonal Antibody (Product # PA1-24462) LEF1 Polyclonal Antibody at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LEF1 was performed by loading tranfected 293T cells. Proteins were transferred to a membrane and probed with a LEF1 Polyclonal Antibody (Product # PA1-24462) at a dilution of 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-LEF1 Polyclonal Antibody (Product # PA1-24462) and a 45, 55 kDa band corresponding to LEF1 was observed across the panel tested except Caco-2 and HeLa which are reported to be negative. Fresh Nuclear enriched extracts (40 µg lysate) of MOLT-4 (Lane 1), Jurkat (Lane 2), Caco-2 (Lane 4), HeLa (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Few uncharacterized bands (*) were observed in HeLa.
