Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF7647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal LEF1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LEF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) TCF-1, rhTCF-2, and rhTCF-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The osteogenic differentiation of human adipose-derived stem cells is regulated through the let-7i-3p/LEF1/β-catenin axis under cyclic strain.
Luo Y, Ge R, Wu H, Ding X, Song H, Ji H, Li M, Ma Y, Li S, Wang C, Du H
Stem cell research & therapy 2019 Nov 21;10(1):339
Stem cell research & therapy 2019 Nov 21;10(1):339
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human LEF1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human LEF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7647) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEF1 at approximately 35-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.