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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ARP32403_T100 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP32403_T100, RRID:AB_842312
- Product name
- LEF1 antibody - C-terminal region (ARP32403_T100)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the C terminal region of human LEF1
- Description
- Protein A purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis using Anti-LEF1 antibody detects protein at predicted molecular weight using Jurkat Cells as a positive control. Tissue/Lysate Concentration: 2.0ug/ml Antibody Concentration: 1.0ug/ml
- Sample type
- Jurkat Cells
- Validation comment
- Western blot analysis detects protein at predicted molecular weight
- Primary Ab dilution
- 1.0ug/ml
- Other comments
- Lysate concentration 2.0ug/ml
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Human Intestine with Anti-LEF1 antibody
- Sample type
- Human Intestine
- Validation comment
- This location is supported by literature
- Primary Ab dilution
- 4.0-8.1068
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 0.5ug/mL 0.2ug/mL
- Protocol
- Protocol
