PAB29327

antibody from Abnova Corporation

Targeting: LEF1 TCF10, TCF1ALPHA, TCF7L3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29327

Provider

Abnova Corporation

Product name

LEF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human LEF1.

Antigen sequence

SDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPP
LGWQGQPVYPITGGFRQPYPSSLSVDTSMSRFSHH
MIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLM
HVKPQHEQRKEQEPKRPHIKKPLNAFMLY


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate); Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with LEF1 polyclonal antibody (Cat# PAB29327) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with LEF1 polyclonal antibody (Cat# PAB29327) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with LEF1 polyclonal antibody (Cat# PAB29327) shows strong nuclear positivity in non-germinal center cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)