Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00083PU-N - Provider product page
- Provider
- OriGene
- Product name
- LEF1 mouse monoclonal antibody, clone 2D12, Purified
- Antibody type
- Monoclonal
- Description
- LEF1 mouse monoclonal antibody, clone 2D12, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LEF1
- Isotype
- IgG
- Antibody clone number
- 2D12
- Vial size
- 100 µg
- Concentration
- 0.1mg/ml
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Detection of endogenous LEF Whole cell lysates of serum starved Jurkat were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 2D12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
- Validation comment
- WB