AM05260PU-L

antibody from OriGene

Targeting: LEF1 TCF10, TCF1ALPHA, TCF7L3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AM05260PU-L

Provider

OriGene

Product name

LEF1 mouse monoclonal antibody, clone REMB1, Purified

Antibody type

Monoclonal

Description

LEF1 mouse monoclonal antibody, clone REMB1, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

LEF1

Isotype

IgG

Antibody clone number

REMB1

Vial size

250 µg

Concentration

Lot specific

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using LEF-1 Antibody (Clone REMB1) on Jurkat cell lysate at 5 ug/ml.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF-1. Cells were stained with LEF-1 Antibody (Clone REMB1) and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1.

Validation comment

IF