PA5-82000

antibody from Invitrogen Antibodies

Targeting: LEF1 TCF10, TCF1ALPHA, TCF7L3

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-82000

Provider

Invitrogen Antibodies

Product name

LEF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SDVNSKQGMS RHPPAPDIPT FYPLSPGGVG QITPPLGWQG QPVYPITGGF RQPYPSSLSV DTSMSRFSHH MIPGPPGPHT TGIPHPAIVT PQVKQEHPHT DSDLMHVKPQ HEQRKEQEPK RPHIKKPLNA FMLY

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Changes in the Expression of Smooth Muscle Cell-Related Genes in Human Dermal Sheath Cup Cells Associated with the Treatment Outcome of Autologous Cell-Based Therapy for Male and Female Pattern Hair Loss.

Yoshida Y, Takahashi M, Yamanishi H, Nakazawa Y, Kishimoto J, Ohyama M
International journal of molecular sciences 2022 Jun 27;23(13)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of LEF1 in U-251 MG cells using a LEF1 polyclonal antibody (Product # PA5-82000). The analysis shows localization to nucleoplasm & cytosol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Effect of DPAC medium and CHIR99021 treatment on DSC cells. ( A , B ) Correlation between the treatment outcome score at 3, 6, 9, and 12 months vs. day 0 (3 M/0 M, 6 M/0 M, 9 M/0 M, and 12 M/0 M) or vs. placebo (3 M/p, 6 M/p, 9 M/p, and 12 M/p) during the clinical study using DSC cell lines [], and the expression levels of DP markers. Each value represents the correlation coefficient. Red: positive correlation; blue: inverse correlation. ( C ) Relative expression levels of Sox2, LEF1, Noggin, and BMP4 in three DSC cell lines (DSC#1-#3) cultured in medium (control) or with 3 uM CHIR99021 ( n = 3). Expression levels were normalized to GAPDH and the results are expressed as the mean +- S.D. Student's t -test, * p < 0.05, ** p < 0.01, *** p < 0.001. ( D ) Immunostaining of DSC cells cultured in control or CHIR99021-containing medium for SOX2 (red), LEF1 (green), and beta-catenin (red). Blue indicates Hoechst 33,342 nuclear staining. Arrowheads: beta-catenin nuclear translocation. Bars = 50 um.