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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-31791 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LEF1 Monoclonal Antibody (1A4B11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A4B11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LEF1 in Jurkat (1), HepG2 (2), and MOLT4 (3) cell lysate. Samples were incubated with LEF1 monoclonal antibody (Product # MA5-31791) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LEF1 in human LEF1 recombinant protein. Sample was incubated with LEF1 monoclonal antibody (Product # MA5-31791) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LEF1 in HEK293 (1) and LEF1 cell lysate. Samples were incubated with LEF1 monoclonal antibody (Product # MA5-31791) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of LEF1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR660502_LV and CRISPR660521_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of LEF1 was performed by loading 30 µg of Jurkat wild type (Lane 1) and Jurkat LEF1 KO (Lane 2) modified whole cell extracts. The blot was probed with Anti-LEF1 Monoclonal Antibody (1A4B11)(Product # MA5-31791) using 1:1000 dilution and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to LEF1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of LEF1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with LEF1 monoclonal antibody (Product # MA5-31791) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of KPNA2 in Hela cells (green). Sample was incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LEF1 in paraffin-embedded colon cancer tissue. Sample was incubated with LEF1 monoclonal antibody (Product # MA5-31791) using a dilution of 1:200-1:1000 followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LEF1 in paraffin-embedded esophageal cancer tissue. Sample was incubated with LEF1 monoclonal antibody (Product # MA5-31791) using a dilution of 1:200-1:1000 followed by DAB staining.
