Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-86960 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86960, RRID:AB_11015014
- Product name
- Rabbit Polyclonal LEF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human LEF1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma.
Asplund A, Gry Björklund M, Sundquist C, Strömberg S, Edlund K, Ostman A, Nilsson P, Pontén F, Lundeberg J
The British journal of dermatology 2008 Mar;158(3):527-38
The British journal of dermatology 2008 Mar;158(3):527-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in human cell lines SK-MEL-30 and A-549 using anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEF1 Antibody [NBP1-86960] - Analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human lymph node shows moderate to strong nuclear positivity in non - germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human tonsil shows moderate nuclear positivity in non - germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human pancreas shows no nuclear positivity as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining in human tonsil and pancreas tissues . Corresponding LEF1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LEF1 Antibody [NBP1-86960] - Staining of human oral mucosa shows moderate nuclear positivity in subset of squamous epithelial cells.