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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- Ab112963 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant LEF1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human LEF1 Antibody, Recombinant, could be used for WB, ICC, IF and so on.Application:WB: 1/500 - 1/1000ICC/IF: 1/50 - 1/200Protein FunctionParticipates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 269-282- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant LEF1 Antibody (Ab112963) - Western Blot All lanes: Recombinant LEF1 Antibody (Ab112963) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 25-58 kDa Exposure time: 150.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant LEF1 Antibody (Ab112963) - Western Blot All lanes: Recombinant LEF1 Antibody (Ab112963) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 44 kDa Observed band size: 25-58 kDa Exposure time: 150.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant LEF1 Antibody (Ab112963) - IF/ICC Immunocytochemistry analysis of LEF1 (green) in Jurkat cells using Recombinant LEF1 Antibody (Ab112963), and cells were counterstained with DAPI (blue).
