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Western blot
Immunocytochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- HPA002087 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA002087, RRID:AB_1079245
- Product name
- Anti-LEF1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human LEF1, Gene description: lymphoid enhancer-binding factor 1, Alternative Gene Names: TCF10, TCF1ALPHA, TCF7L3, Validated applications: WB, IHC, ICC, ChIP, Uniprot ID: Q9UJU2, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references NRARP displays either pro- or anti-tumoral roles in T-cell acute lymphoblastic leukemia depending on Notch and Wnt signaling
Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma
Pinto I, Duque M, Gonçalves J, Akkapeddi P, Oliveira M, Cabrita R, Yunes J, Durum S, Barata J, Fragoso R
Oncogene 2019;39(5):975-986
Oncogene 2019;39(5):975-986
Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma
Asplund A, Gry Björklund M, Sundquist C, Strömberg S, Edlund K, Östman A, Nilsson P, Pontén F, Lundeberg J
British Journal of Dermatology 2008;158(3):527-538
British Journal of Dermatology 2008;158(3):527-538
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402529).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-LEF1 (HPA002087, Lot 000050271) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
