Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00083PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00083PU-N, RRID:AB_1004553
- Product name
- anti LEF1
- Antibody type
- Monoclonal
- Antigen
- Recombinant LEF
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D12
- Vial size
- 0.1 mg
- Concentration
- 0.1mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of endogenous LEF Whole cell lysates of serum starved Jurkat were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 2D12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).