Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN953158 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (N-Term), (AA 17-45) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human LEF1
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 17-45
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references LEF-1 is a prosurvival factor in chronic lymphocytic leukemia and is expressed in the preleukemic state of monoclonal B-cell lymphocytosis.
Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements.
A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts.
Gutierrez A Jr, Tschumper RC, Wu X, Shanafelt TD, Eckel-Passow J, Huddleston PM 3rd, Slager SL, Kay NE, Jelinek DF
Blood 2010 Oct 21;116(16):2975-83
Blood 2010 Oct 21;116(16):2975-83
Human CD1D gene expression is regulated by LEF-1 through distal promoter regulatory elements.
Chen QY, Zhang T, Pincus SH, Wu S, Ricks D, Liu D, Sun Z, Maclaren N, Lan MS
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5047-54
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5047-54
A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts.
Kalsi G, Kuo PH, Aliev F, Alexander J, McMichael O, Patterson DG, Walsh D, Zhao Z, Schuckit M, Nurnberger J Jr, Edenberg H, Kramer J, Hesselbrock V, Tischfield JA, Vladimirov V, Prescott CA, Dick DM, Kendler KS, Riley BP
Human molecular genetics 2010 Jun 15;19(12):2497-506
Human molecular genetics 2010 Jun 15;19(12):2497-506
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of LEF1 Antibody (N-term) Cat.-No AP52463PU-N in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the LEF1 antibody detected the LEF1 protein (arrow).