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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- TA804474 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA804474, RRID:AB_2627322
- Product name
- TCF12 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6)
- Antibody type
- Monoclonal
- Description
- TCF12 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TCF12
- Isotype
- IgG
- Antibody clone number
- OTI4D6
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCF12 (RC220164, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TCF12.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-TCF12 monoclonal antibody. (1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TCF12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804474)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TCF12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804474)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TCF12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804474)
- Validation comment
- IHC
