LS-C376758

antibody from LSBio

Targeting: TCF12 bHLHb20, HEB, HsT17266, HTF4, p64

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C376758

Provider

LSBio

Product name

TCF12 / HEB Antibody (aa443-682) LS-C376758

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating TCF12 in Jurkat whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of TCF12 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: TCF12 Antibody (4µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating TCF12 in Jurkat whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of TCF12 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: TCF12 Antibody (4µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating TCF12 in Jurkat whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of TCF12 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: TCF12 Antibody (4µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (20µg)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution.